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Mouse Adiponectin (ADP) ELISA Kit

CAT: 0013-GTR18864265-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18864265-01Size:48 Tests
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Description
Mouse Adiponectin (ADP) ELISA Kit
Product Name Alternative
GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
UniProt
Q60994
Reactivity
Mouse
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
4.75 ng/mL
Notes
For research use only.
Applications Notes
Standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ADP in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
7.82-500 ng/mL

UniProtKB · Q60994

Adiponectin

ADIPO_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q60994
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Adipoq
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q62400, Q6GTX4, Q9DC68
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Domain

CollagenRepeatSignal

PTM

Disulfide bondGlycoproteinHydroxylation

Molecular function

Hormone

Cellular component

Secreted

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