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Human Complement Component 3a (C3a) ELISA Kit

CAT: 0013-GTR18864189-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18864189-01Size:48 Tests
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Description
Human Complement Component 3a (C3a) ELISA Kit
Product Name Alternative
Complement Component 3a
UniProt
P01024
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
2.12 ng/mL
Notes
For research use only.
Applications Notes
Standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3a in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
7.81-500 ng/mL

UniProtKB · P01024

Complement C3

CO3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01024
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A7E236
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Age-related macular degenerationDisease variantHemolytic uremic syndrome

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoproteinThioester bond

Biological process

Complement alternate pathwayComplement pathwayFatty acid metabolismImmunityInflammatory responseInnate immunityLipid metabolism

Cellular component

Secreted

Domain

Signal

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