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Mouse Complement Component 3a (C3a) ELISA Kit

CAT: 0013-GTR18854996-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854996-01Size:48 Tests
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Description
Mouse Complement Component 3a (C3a) ELISA Kit
Product Name Alternative
C3a-desArg, ASP
UniProt
P01027
Reactivity
Mouse
Target
C3a
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
0.10 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C3a. You can calculate the concentration of Mouse C3a in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10ng/mL

UniProtKB · P01027

Complement C3

CO3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P01027
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q61370, Q80XP1
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Coding sequence diversity

Alternative initiation

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoproteinThioester bond

Biological process

Complement alternate pathwayComplement pathwayFatty acid metabolismImmunityInflammatory responseInnate immunityLipid metabolism

Cellular component

Secreted

Domain

Signal

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