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Mouse Protein C (PROC) ELISA Kit

CAT: 0013-GTR18863973-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863973-01Size:48 Tests
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Description
Mouse Protein C (PROC) ELISA Kit
Product Name Alternative
PRO-C; VKDPC; PC; Vitamin K Dependent Protein C; Inactivator Of Coagulation Factors Va And VIIIa; Autoprothrombin IIA; Blood coagulation factor XIV; Activation peptide
UniProt
P33587
Reactivity
Mouse
Field of Research
Cell Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Plasma
Sensitivity
0.052 μg/mL
Notes
For research use only.
Applications Notes
Standard: 10 μg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PROC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PROC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PROC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PROC in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.16-10 μg/mL

UniProtKB · P33587

Vitamin K-dependent protein C

PROC_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P33587
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Proc
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.21.69
Processing
Precursor
Secondary accessions
O35498, Q91WN8, Q99PC6
Protein keywords

Biological process

Blood coagulationHemostasis

Ligand

Calcium

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acidGlycoproteinHydroxylationZymogen

Domain

EGF-like domainRepeatSignal

Cellular component

Endoplasmic reticulumGolgi apparatusSecreted

Molecular function

HydrolaseProteaseSerine protease

Technical term

Reference proteome

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