PROC ELISA Kit (Mouse)

CAT: 0247-OKCD02823-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02823-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Protein C (PROC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Protein C (PROC) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Protein C
Gene Aliases
Anticoagulant protein C; autoprothrombin IIA; blood coagulation factor XIV; inactivator of coagulation factors Va, VIII; P; PC; vitamin K-dependent protein C.
Gene ID
19123
Accession Number
NP_001036232.1
Reactivity
Mouse|Mus musculus
Target
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein C (PROC) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein C (PROC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein C (PROC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein C (PROC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Plasma
Detection Range
15.6-1,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) sodium citrate plasma (n=5) 97-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) sodium citrate plasma (n=5) 97-105 101 sodium citrate plasma (n=5) 97-105 101
Sensitivity
6.9 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-PROC Microplate 96 Wells (12 x 8 Well strips) PROC Lyophilized Standard 2 100X Biotinylated PROC Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-PROC Microplate 96 Wells (12 x 8 Well strips) Anti-PROC Microplate 96 Wells (12 x 8 Well strips) PROC Lyophilized Standard 2 PROC Lyophilized Standard 2 100X Biotinylated PROC Detector Antibody 1 x 120 uL 100X Biotinylated PROC Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Protein C (PROC) . No significant cross-reactivity or interference between Protein C (PROC) and analogues was observed.
Protein Length
460
NCBI Gene Symbol
Proc
Protein Name
Vitamin K-dependent protein C
Gene Name URL
Proc
Nucleotide Accession Number
NM_001042767.3

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