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Mouse Complement Factor D (CFD) ELISA Kit

CAT: 0013-GTR18863967-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863967-01Size:48 Tests
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Description
Mouse Complement Factor D (CFD) ELISA Kit
Product Name Alternative
CF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator
UniProt
P03953
Reactivity
Mouse
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity
0.057 μg/mL
Notes
For research use only.
Applications Notes
Standard: 10 μg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CFD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CFD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CFD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CFD in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.16-10 μg/mL

UniProtKB · P03953

Complement factor D

CFAD_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P03953
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cfd
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.21.46
Processing
Precursor
Secondary accessions
Q61280
Protein keywords

Technical term

3D-structureReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Complement alternate pathwayImmunityInnate immunity

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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