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Rat Complement Factor D (CFD) ELISA Kit

CAT: 0013-GTR18854022-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854022-01Size:48 Tests
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Description
Rat Complement Factor D (CFD) ELISA Kit
Product Name Alternative
ADN, Adipsin, DF, PFD, Factor D, Properdin Factor D Esterase
UniProt
P32038
Reactivity
Rat
Target
CFD
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
75.00 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CFD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CFD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CFD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CFD. You can calculate the concentration of Rat CFD in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
125-8000ng/mL

UniProtKB · P32038

Complement factor D

CFAD_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P32038
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cfd
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
3.4.21.46
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Complement alternate pathwayImmunityInnate immunity

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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