Products for Research Use Only

Mouse Complement Component 5 (C5) ELISA Kit

CAT: 0013-GTR18863960-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863960-01Size:48 Tests
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Description
Mouse Complement Component 5 (C5) ELISA Kit
Product Name Alternative
CPAMD4; C5a anaphylatoxin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
UniProt
P06684
Reactivity
Mouse
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
0.069 μg/mL
Notes
For research use only.
Applications Notes
Standard: 10 μg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C5 in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.16-10 μg/mL

UniProtKB · P06684

Complement C5

CO5_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P06684
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C5
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A2AS36
Protein keywords

Technical term

3D-structureReference proteome

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Biological process

Complement alternate pathwayComplement pathwayCytolysisImmunityInflammatory responseInnate immunity

Cellular component

MembraneMembrane attack complexSecretedTarget cell membraneTarget membrane

Domain

Signal