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Rat Complement Component 5 (C5) ELISA Kit

CAT: 0013-GTR18856761-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18856761-01Size:48 Tests
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Description
Rat Complement Component 5 (C5) ELISA Kit
Product Name Alternative
CPAMD4
UniProt
P08650
Reactivity
Rat
Target
C5
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
0.19 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C5. You can calculate the concentration of Rat C5 in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20ng/mL

UniProtKB · P08650

Complement C5

CO5_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P08650
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C5
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A0A096P6L9, Q63078
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Biological process

Complement alternate pathwayComplement pathwayCytolysisImmunityInflammatory responseInnate immunity

Cellular component

MembraneMembrane attack complexSecretedTarget cell membraneTarget membrane

Domain

Signal

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