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P4HA1 Antibody

CAT: 0013-GTR17697174Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17697174Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to P4HA1
Product Name Alternative
Anti P4HA1 antibody, anti procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I antibody, anti 4-PH alpha-1 antibody, anti P4HA antibody, anti prolyl 4-hydroxylase, alpha I subunit antibody
Reactivity
Human
Target
P4HA1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DFARKDEPDAFKE
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, IF/ICC: 10μg/ml
Molecular Weight
61.0, 61.0
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in lysates of cell lines HEK293, A431 and NIH3T3 (calculated MW of 61.0kDa according to NP_000908.2 and NP_001017962.1) . Recommended concentration: 0.1-0.1 μg/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, IF, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
5033

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