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Mouse Ferritin (FE) ELISA Kit

CAT: 0013-GTR18863827-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863827-01Size:48 Tests
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Description
Mouse Ferritin (FE) ELISA Kit
Product Name Alternative
Ferritin
UniProt
P29391
Reactivity
Mouse
Field of Research
Cancer Biology, Hematology, Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.112 ng/mL
Notes
For research use only.
Applications Notes
Standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FE in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
7.81-500 ng/mL

UniProtKB · P29391

Ferritin light chain 1

FRIL1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P29391
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ftl1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q8WUQ8
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Cellular component

CytoplasmCytoplasmic vesicleLysosome

Ligand

IronMetal-binding

Biological process

Iron storage

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