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Human Complement Component 2 (C2) ELISA Kit

CAT: 0013-GTR18863430-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863430-01Size:48 Tests
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Description
Human Complement Component 2 (C2) ELISA Kit
Product Name Alternative
CO2; C3/C5 convertase
UniProt
P06681
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
3.2 ng/mL
Notes
For research use only.
Applications Notes
Standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C2 in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
7.82-500 ng/mL

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