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Pig Lactoferrin (LTF) ELISA Kit

CAT: 0013-GTR18863718-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863718-01Size:48 Tests
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Description
Pig Lactoferrin (LTF) ELISA Kit
Product Name Alternative
HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1
UniProt
P14632
Reactivity
Porcine
Field of Research
Neuroscience; Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
19.3 ng/mL
Notes
For research use only.
Applications Notes
Standard: 5000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig LTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig LTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig LTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig LTF in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
78.13-5000 ng/mL

UniProtKB · P14632

Lactotransferrin

TRFL_PIG · Sus scrofa

View on UniProt ↗
Primary accession
P14632
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
LTF
Protein existence
1: Evidence at protein level
Organism
Sus scrofa (Pig)
Taxonomy ID
9823
Alternative names
Lactoferrin
EC number
3.4.21.-
Processing
Precursor
Secondary accessions
Q29557
Protein keywords

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoprotein

Molecular function

HydrolaseProteaseSerine protease

Biological process

ImmunityIon transportIron transportOsteogenesisTransport

Ligand

IronMetal-binding

Domain

RepeatSignal

Cellular component

Secreted

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