Rabbit anti Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

Rabbit anti Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

• Background: GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots. Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
• CAS Number: 9007-83-4
• UniProt: P04406
• Host: Rabbit
• Species Reactivity: Human
• Isotype: IgG
• Conjugation: Affinity Purified
• Type: Antigen Immunoaffinity Purified Polyclonal
• Applications: WB, ELISA
• Field of Research: Signal Transduction
• Purification Method: Antigen Immunoaffiinity Purification
• Assay Principle: Antibody can be used for Western Blotting (1:400 dilution) and immunohistochemistry (2-10 µg/mL). Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Nakajima, H., et al. 'GAPDH aggregate formation participates in oxidative stress-induced cell death.' J. Biol. Chem. 2009:Epub 2. Nahlik, K.W., et al. 'Modulation of GAPDH expression and cellular localization after vaccinia virus infection of Human adherent monocytes.' Acta Biochim. Pol. 2003: 50(3); 667-676.
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB