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Human Integrin alpha-M (Itgam/CD11b) ELISA Kit

CAT: 0013-GTR18857285-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18857285-01Size:48 Tests
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Description
Human Integrin alpha-M (Itgam/CD11b) ELISA Kit
Product Name Alternative
CD11B, CR3A, MAC-1, MAC1A, MGC11744, MO1A, SLEB6, antigen CD11b (p17) |integrin alpha M|macrophage antigen alpha polypeptide|neutrophil adherence receptor alpha-M subunit
UniProt
P11215
Reactivity
Human
Target
Itgam/CD11b
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
0.38 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Itgam/CD11b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Itgam/CD11b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Itgam/CD11b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Itgam/CD11b. You can calculate the concentration of Human Itgam/CD11b in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.63-40ng/mL

UniProtKB · P11215

Integrin alpha-M

ITAM_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P11215
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ITGAM
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q4VAK0, Q4VAK1, Q4VAK2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumMagnesiumMetal-binding

Biological process

Cell adhesionImmunityInnate immunity

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoprotein

Molecular function

IntegrinReceptor

Domain

RepeatSignalTransmembraneTransmembrane helix

Disease

Systemic lupus erythematosus

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