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Human Eukaryotic Translation Initiation Factor 2 Alpha (EIF2a) ELISA Kit

CAT: 0013-GTR18857033-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18857033-01Size:48 Tests
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Description
Human Eukaryotic Translation Initiation Factor 2 Alpha (EIF2a) ELISA Kit
Product Name Alternative
EIF2A, EIF-2A, MST89, MSTP4, MSTP89, CDA2, eukaryotic translation initiation factor 2A
UniProt
Q9BY44
Reactivity
Human
Target
EIF2a
Field of Research
Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
46.88 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EIF2a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EIF2a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EIF2a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human EIF2a. You can calculate the concentration of Human EIF2a in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
78.13-5000pg/mL

UniProtKB · Q9BY44

Eukaryotic translation initiation factor 2A

EIF2A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BY44
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EIF2A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
eIF-2A
EC number
—
Processing
—
Secondary accessions
A8MPS6, B4DF96, B4DQ14, D3DNI9, Q5QTR2, Q7Z4E9, Q8NFM1, Q96EW9, Q96K81
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Domain

Coiled coilRepeatWD repeat

Molecular function

Initiation factor

Biological process

Protein biosynthesisTranslation regulation

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