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Mouse Apobec 1 Complementation Factor (ACF) ELISA Kit

CAT: 0013-GTR18856541-02Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18856541-02Size:96 Tests
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Description
Mouse Apobec 1 Complementation Factor (ACF) ELISA Kit
Product Name Alternative
ACF, ASP, A1CF, APOBEC1-stimulating protein
UniProt
Q5YD48
Reactivity
Mouse
Target
ACF
Field of Research
Cell Biology
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
18.75 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ACF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ACF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ACF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ACF. You can calculate the concentration of Mouse ACF in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
31.25-2000pg/mL

UniProtKB · Q5YD48

APOBEC1 complementation factor

A1CF_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q5YD48
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
A1cf
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
E9QJS8, Q5FW55, Q5YD47
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmEndoplasmic reticulumNucleus

Biological process

mRNA processing

Technical term

Reference proteome

Domain

Repeat

Molecular function

RNA-binding

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