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HSP70 ELISA Kit (High-Sensitivity)

CAT: 0013-GTR18855589Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18855589Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
High-Sensitivity Colorimetric detection of HSP70
Product Name Alternative
HSP70 1, HSP70 2, HSP70.1, HSP72, HSPA1, HSPA1A, HSPA1B
Reactivity
Canine, Human, Monkey, Mouse, Rat
Target
HSP70 ELISA Kit (High-Sensitivity)
Field of Research
Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction
Assay Type
Sandwich
Sample Type
Plasma, Cell Lysates, Tissue
Sensitivity
0.038 ng/ml
Storage Conditions
4°C and -20°C
Notes
For research use only.
Applications Notes
1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 μL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour while shaking at 600rpm. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 μL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at at 37°C for 1 hour while shaking at 600rpm. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 μL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at 37°C for 30 minutes while shaking at 600rpm. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 μL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 μL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Dynamic Range
0.42 - 27 ng/ml

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