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Nitrotyrosine ELISA Kit

CAT: 0013-GTR18855586Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18855586Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Colorimetric detection of Nitrotyrosine
Product Name Alternative
3 Nitrotyrosine, 3-NT, 3-Nitro-L-tyrosine, 3-Nitrotyrosine, 3NT, Nitro tyrosine
Reactivity
All
Target
Nitrotyrosine ELISA Kit
Field of Research
Cancer Biology, Cell Biology, Neuroscience, Protein Biochemistry, Signal Transduction
Assay Type
Competitive
Sample Type
Plasma, Serum, Cell Lysates, Urine
Sensitivity
50 nM
Storage Conditions
4°C and -20°C
Notes
For research use only.
Applications Notes
1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 μL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 μL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 μL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 μL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.
Dynamic Range
62.5 - 8000 nM

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