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Mouse Basic Fibroblast Growth Factor (bFGF/FGF2) ELISA Kit

CAT: 0013-GTR18855004-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18855004-01Size:48 Tests
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Description
Mouse Basic Fibroblast Growth Factor (bFGF/FGF2) ELISA Kit
Product Name Alternative
FGF-2, B-FGF, BFGF, FGFB, HBGF-2, prostatropin, heparin-binding growth factor 2
UniProt
P15655
Reactivity
Mouse
Target
BFGF/FGF2
Field of Research
Neuroscience; Cardiovascular Research; Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
9.38 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse bFGF/FGF2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse bFGF/FGF2. You can calculate the concentration of Mouse bFGF/FGF2 in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000pg/mL

UniProtKB · P15655

Fibroblast growth factor 2

FGF2_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P15655
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Fgf2
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
FGF-2
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

AngiogenesisDifferentiation

Molecular function

Developmental proteinGrowth factorHeparin-bindingMitogen

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Cellular component

NucleusSecreted

Technical term

Reference proteome

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