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Mouse Prolactin (PRL) ELISA Kit

CAT: 0013-GTR18854792-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854792-01Size:48 Tests
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Description
Mouse Prolactin (PRL) ELISA Kit
Product Name Alternative
Lactotrope, LTH, Luteotropic Hormone
UniProt
P06879
Reactivity
Mouse
Target
PRL
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
0.47 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PRL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PRL. You can calculate the concentration of Mouse PRL in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.78-50ng/mL

UniProtKB · P06879

Prolactin

PRL_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P06879
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Prl
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
PRL
EC number
—
Processing
Precursor
Secondary accessions
Q544X8
Protein keywords

PTM

Disulfide bondPhosphoprotein

Molecular function

Hormone

Biological process

Lactation

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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