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Human Complement Component 3 (C3) ELISA Kit

CAT: 0013-GTR18854597-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854597-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human Complement Component 3 (C3) ELISA Kit
Product Name Alternative
ASP, CPAMD1, AHUS5, ARMD9, C3a, C3b
UniProt
P01024
Reactivity
Human
Target
C3
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, cerebrospinal fluid
Sensitivity
0.59 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C3. You can calculate the concentration of Human C3 in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
1.56-100ng/mL

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