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Human Midkine (MK) ELISA Kit

CAT: 0013-GTR18854369-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854369-01Size:48 Tests
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Description
Human Midkine (MK) ELISA Kit
Product Name Alternative
Midkine , MK , MDK, neurite growth-promoting factor 2, NEGF2, ARAP
UniProt
P21741
Reactivity
Human
Target
MK
Field of Research
Cardiovascular Research, Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Tissue homogenate, Cell lysate, Cell culture supernatant
Sensitivity
0.10 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MK. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MK. You can calculate the concentration of Human MK in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10ng/mL

UniProtKB · P21741

Midkine

MK_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P21741
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MDK
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MK
EC number
—
Processing
Precursor
Secondary accessions
Q2LEK4, Q9UCC7
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

Developmental proteinGrowth factorHeparin-bindingMitogen

Biological process

Differentiation

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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