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Rat Klotho (KL) ELISA Kit

CAT: 0013-GTR18853916-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853916-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This kit is designed for detection of soluble alpha-Klotho in rat samples.
Product Name Alternative
KL
UniProt
Q9Z2Y9
Reactivity
Rat
Target
KL
Field of Research
Musculoskeletal & Connective Tissue Research, Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
0.10 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat KL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat KL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat KL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat KL. You can calculate the concentration of Rat KL in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10ng/mL

UniProtKB · Q9Z2Y9

Klotho

KLOT_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q9Z2Y9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Kl
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
3.2.1.31
Processing
Precursor
Secondary accessions
—
Protein keywords

Cellular component

Cell membraneMembraneSecreted

PTM

Glycoprotein

Molecular function

GlycosidaseHydrolase

Technical term

Reference proteome

Domain

RepeatSignalTransmembraneTransmembrane helix

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