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Human Klotho (KL) ELISA Kit

CAT: 0013-GTR18809223-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18809223-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Klotho (KL) .
Product Name Alternative
Kl , KLOT, Klotho peptide
UniProt
Q9UEF7
Reactivity
Human
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.054 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KL in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL

UniProtKB · Q9UEF7

Klotho

KLOT_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9UEF7
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KL
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.2.1.31
Processing
Precursor
Secondary accessions
Q5VZ95, Q96KV5, Q96KW5, Q9UEI9, Q9Y4F0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

Disease

Disease variant

PTM

Glycoprotein

Molecular function

GlycosidaseHormoneHydrolase

Domain

RepeatSignalTransmembraneTransmembrane helix

Ligand

Vitamin D

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