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Human Renin (REN) Microsample Fast ELISA Kit

CAT: 0013-GTR18853658-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853658-01Size:48 Tests
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Description
Human Renin (REN) Microsample Fast ELISA Kit
Product Name Alternative
REN1, HNFJ2, renin-1
UniProt
P00797
Reactivity
Human
Target
REN
Field of Research
Cancer Biology, Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples; Sample volume:25μL
Sensitivity
18.75 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human REN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human REN. You can calculate the concentration of Human REN in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
31.25-2000pg/mL

UniProtKB · P00797

Renin

RENI_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P00797
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
REN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.23.15
Processing
Precursor
Secondary accessions
Q6FI38, Q6T5C2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

Aspartyl proteaseHydrolaseProtease

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinZymogen

Disease

Disease variant

Cellular component

MembraneSecreted

Domain

Signal

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