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Mouse Renin (REN) Microsample Fast ELISA Kit

CAT: 0013-GTR18853635-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853635-01Size:48 Tests
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Description
Mouse Renin (REN) Microsample Fast ELISA Kit
Product Name Alternative
REN1, HNFJ2, renin-1
UniProt
P06281
Reactivity
Mouse
Target
REN
Field of Research
Cancer Biology, Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples; Sample volume:25μL
Sensitivity
0.1 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse REN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse REN. You can calculate the concentration of Mouse REN in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10ng/mL

UniProtKB · P06281

Renin-1

RENI1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P06281
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ren1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.23.15
Processing
Precursor
Secondary accessions
P97911, Q543E5, Q62153, Q62154
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Molecular function

Aspartyl proteaseHydrolaseProtease

PTM

Disulfide bondGlycoproteinZymogen

Cellular component

MembraneSecreted

Domain

Signal

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