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Rat Melatonin (MT) ELISA Kit

CAT: 0013-GTR18853180-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853180-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Rat Melatonin (MT) ELISA Kit
Product Name Alternative
N-Acetyl-5-Methoxytryptamine
Reactivity
Rat
Target
MT
Field of Research
Pharmacology & Drug Discovery
Assay Type
Competitive
Assay Performance Time
2.5h
Sample Type
Cell lysates and other biological fluids
Sensitivity
2.78 pg/mL
Notes
For research use only.
Applications Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MT in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Applications
ELISA
Dynamic Range
7.82-500 pg/mL

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