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Human Cyclin E1 (CCNE1) ELISA Kit

CAT: 0013-GTR18853071-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853071-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCNE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCNE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCNE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCNE1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
3938; BG:DS07108.3; br37; cdi7; Cdi7; CDI7; CG3938; Cyc; Cyc E; Cyc-E; cycE; CYCE; CycEI; CyclE; cyclin E; cyclin-E; cycline; cyclinE; CyE-1; CyeE; D-CycE; dCycE; dm-cycE; DmcycE; DmCycE; DmcyclinE; Dmel\CG3938; fond; l (2) 05206; l (2) 35Dd; l (2) br37; l (2) k02514; l (2) k02602; l (2) k05007; l35Dd
UniProt
P24864
Reactivity
Human
Field of Research
Cancer Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
29.7 pg/mL
Notes
For research use only.
Applications Notes
Standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCNE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCNE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCNE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCNE1 in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
78.13-5000 pg/mL

UniProtKB · P24864

G1/S-specific cyclin-E1

CCNE1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P24864
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CCNE1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A8K684, Q14091, Q8NFG1, Q92501, Q9UD21
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell cycleCell division

Molecular function

Cyclin

Cellular component

Nucleus

PTM

PhosphoproteinUbl conjugation

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