Anti-Hu CD266 APC

• Description:

  CD266 / TWEAK R (TNFRSF12A), also known as FN14 (fibroblast growth factor-inducible 14) is a receptor for CD255 / TWEAK, the TNF-like weak inducer of apoptosis. CD266 is expressed on endothelial cells, as well as on some cancer tissues, and plays a role in CD255-induced endothelial cell migration, proliferation, and angiogenesis. The CD255-CD266 interaction, or antibody-mediated triggering of CD266 is also able to induce apoptosis and necrosis in CD266-positive cells (including tumor cells), which might have therapeutic potential.

• Certification:

  RUO

• Reactivity:

  Mouse, Human

• Immunogen:

  Human CD266-transfected P815 cells

• Target Antigen:

  CD266

• Clone:

  ITEM-4

• Applications:

  FC (QC tested)

• Concentration:

  0.1 mg/mL

• Format:

  APC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Yoriki R, Akashi S, Sho M, Nomi T, Yamato I, Hotta K, Takayama T, Matsumoto S, Wakatsuki K, Migita K, Yagita H, Nakajima Y: Therapeutic potential of the TWEAK/Fn14 pathway in intractable gastrointestinal cancer. Exp Ther Med. 2011 Jan;2 (1) :103-108, URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=yoriki+r+akashi+s , *Sanz AB, Sanchez-Niñ o MD, Carrasco S, Manzarbeitia F, Ruiz-Andres O, Selgas R, Ruiz-Ortega M, Gonzalez-Enguita C, Egido J, Ortiz A: Inflammatory cytokines and survival factors from serum modulate tweak-induced apoptosis in PC-3 prostate cancer cells. PLoS One. 2012;7 (10) :e47440., URL: http://www.ncbi.nlm.nih.gov/pubmed/23077618 , *Nakayama M, Ishidoh K, Kojima Y, Harada N, Kominami E, Okumura K, Yagita H: Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death. J Immunol. 2003 Jan 1;170 (1) :341-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/12496418 , *Roos C, Wicovsky A, Müller N, Salzmann S, Rosenthal T, Kalthoff H, Trauzold A, Seher A, Henkler F, Kneitz C, Wajant H: Soluble and transmembrane TNF-like weak inducer of apoptosis differentially activate the classical and noncanonical NF-kappa B pathway. J Immunol. 2010 Aug 1;185 (3) :1593-605. , URL: http://www.ncbi.nlm.nih.gov/pubmed/20610643

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody ITEM-4 recognizes an extracellular epitope of CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis.

• Applications Notes:

  Flow cytometry: Recommended dilution: 5-15 μg/ml. Extracellular and intracellular staining.

• Isotype:

  Mouse IgG2b