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General Actin Beta (ACTb) ELISA Kit

CAT: 0013-GTR18814165-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18814165-01Size:48 Tests
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Description
General Actin Beta (ACTb) ELISA Kit
Product Name Alternative
ACT-B ELISA Kit, PS1TP5BP1 ELISA Kit, Actin, cytoplasmic 1 ELISA Kit, Actin, cytoplasmic 1, N-terminally processed ELISA Kit
Reactivity
All
Target
ACTb
Field of Research
Cancer Biology; Musculoskeletal & Connective Tissue Research
Assay Type
Competitive
Assay Performance Time
2h
Sample Type
Cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.057 ng/mL
Notes
For research use only.
Applications Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ACTb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ACTb in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL

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