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General Vitamin B2 (VB2) ELISA Kit

CAT: 0013-GTR18813686-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18813686-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
General Vitamin B2 (VB2) ELISA Kit
Product Name Alternative
Riboflavin ELISA Kit, Riboflavine ELISA Kit
Reactivity
All
Target
VB2
Field of Research
Metabolism Research
Assay Type
Competitive
Assay Performance Time
2h
Sample Type
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Sensitivity
2.45 ng/mL
Notes
For research use only.
Applications Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Applications
ELISA
Dynamic Range
9.38-600 ng/mL

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