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Rat Eukaryotic Initiation Factor 4E (eIF4E) ELISA Kit

CAT: 0013-GTR18813905-02Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18813905-02Size:96 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to eIF4E (eukaryotic initiation factor 4E) .
Product Name Alternative
CBP ELISA Kit, eIF 4E ELISA Kit, eIF 4F 25 kDa subunit ELISA Kit, EIF4E ELISA Kit, EIF4E1 ELISA Kit, EIF4EL1 ELISA Kit, EIF4F ELISA Kit
UniProt
P63074
Reactivity
Rat
Field of Research
Cancer Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
26.14 pg/mL
Notes
For research use only.
Applications Notes
Standard: 6000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat eIF4E. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat eIF4E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat eIF4E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat eIF4E in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
93.75-6000 pg/mL

UniProtKB · P63074

Eukaryotic translation initiation factor 4E

IF4E_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P63074
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Eif4e
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
eIF-4E, eIF4E
EC number
—
Processing
—
Secondary accessions
P20415
Protein keywords

PTM

AcetylationPhosphoprotein

Cellular component

CytoplasmNucleus

Molecular function

Initiation factorRNA-binding

Biological process

mRNA transportProtein biosynthesisTranslation regulationTransport

Technical term

Reference proteome

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