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Mouse Noggin (NOG) ELISA Kit

CAT: 0013-GTR18813233-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18813233-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Noggin (NOG) .
Product Name Alternative
SYM1 ELISA Kit, SYNS1 ELISA Kit, Synostoses (Multiple) Syndrome 1 ELISA Kit, Symphalangism 1 (Proximal) ELISA Kit
UniProt
P97466
Reactivity
Mouse
Field of Research
Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.066 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NOG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NOG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NOG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NOG in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL

UniProtKB · P97466

Noggin

NOGG_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P97466
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Nog
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

ChondrogenesisDifferentiation

Molecular function

Developmental protein

PTM

Disulfide bondGlycoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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