Products for Research Use Only

Recombinant Human Noggin (NOG) (Active)

CAT: 0013-GTR19063839-01Size: 10 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR19063839-01Size:10 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
This Recombinant Human Noggin (NOG) (Active) spans the amino acid sequence from region 28-232aa. Purity: Greater than 95% as determined by SDS-PAGE.
Product Name Alternative
Noggin; NOG
UniProt
Q13253
Expression Region
28-232aa
Tag
Tag free
Source
Mammalian cell
Origin
Homo sapiens (Human)
Field of Research
Stem Cell & Developmental Biology
Endotoxin
≤10 EU/mg by the LAL method
Purity
Greater than 95% as determined by SDS-PAGE.
Bioactivity
Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.
Form
Lyophilized powder
Molecular Weight
23 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
Lyophilized from a 0.2 μm filtered solution containingPBS, 5% mannitoland 0.01% Tween 80, pH 7.4
AA Sequence
QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC

UniProtKB · Q13253

Noggin

NOGG_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q13253
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NOG
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

ChondrogenesisDifferentiation

Disease

DeafnessDisease variant

Molecular function

Developmental protein

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal