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Rat Citrate Synthase (CS) ELISA Kit

CAT: 0013-GTR18812860-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18812860-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Citrate Synthase (CS) .
Product Name Alternative
Citrate (Si) -synthase, mitochondrial ELISA Kit
UniProt
Q8VHF5
Reactivity
Rat
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
1.23 ng/mL
Notes
For research use only.
Applications Notes
Standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CS in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
3.13-200 ng/mL

UniProtKB · Q8VHF5

Citrate synthase, mitochondrial

CISY_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q8VHF5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cs
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
2.3.3.1
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

AcetylationMethylationPhosphoproteinUbl conjugation

Technical term

Direct protein sequencingReference proteome

Cellular component

Mitochondrion

Molecular function

Transferase

Domain

Transit peptide

Biological process

Tricarboxylic acid cycle

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