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Mouse Citrate Synthase (CS) ELISA Kit

CAT: 0013-GTR18810665-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18810665-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Citrate Synthase (CS) .
Product Name Alternative
Citrate (Si) -synthase, mitochondrial ELISA Kit
UniProt
Q9CZU6
Reactivity
Mouse
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.62 ng/mL
Notes
For research use only.
Applications Notes
Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CS in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
1.57-100 ng/mL

UniProtKB · Q9CZU6

Citrate synthase, mitochondrial

CISY_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9CZU6
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cs
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
2.3.3.1
Processing
Precursor
Secondary accessions
Q3UDP3
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationMethylationPhosphoproteinUbl conjugation

Cellular component

Mitochondrion

Molecular function

Transferase

Domain

Transit peptide

Biological process

Tricarboxylic acid cycle

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