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Human Norepinephrine Transporter (NET) ELISA Kit

CAT: 0013-GTR18810692-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18810692-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Norepinephrine Transporter (NET) .
Product Name Alternative
SLC6A2 ELISA Kit, NAT1 ELISA Kit, NET ELISA Kit, NET1 ELISA Kit, Sodium-dependent noradrenaline transporter ELISA Kit, Noradrenaline Transporter ELISA Kit, Solute Carrier Family 6 member 2 ELISA Kit, Neurotransmitter transporter ELISA Kit, Noradrenalin ELISA Kit
UniProt
P23975
Reactivity
Human
Field of Research
Pharmacology & Drug Discovery
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.106 ng/mL
Notes
For research use only.
Applications Notes
Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NET. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NET. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NET, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NET in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.32-20 ng/mL

UniProtKB · P23975

Sodium-dependent noradrenaline transporter

SC6A2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P23975
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SLC6A2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R707, B4DX48, Q96KH8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCell projectionMembraneSynapseSynaptosome

Disease

Disease variant

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Ligand

Metal-bindingSodium

Biological process

Neurotransmitter transportSymportTransport

Domain

TransmembraneTransmembrane helix

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