Rabbit Myelin Basic Protein (MBP) ELISA Kit

CAT: 0013-GTR18810685-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0013-GTR18810685-01Size:48 Tests

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin Basic Protein (MBP) .

Product Name Alternative

Myelin A1 protein ELISA Kit, Myelin membrane encephalitogenic protein ELISA Kit

UniProt

P25274

Reactivity

Rabbit

Field of Research

Neuroscience

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma and other biological fluids.

Sensitivity

0.054 ng/mL

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit MBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit MBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit MBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit MBP in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

UniProtKB · P25274

Myelin basic protein

MBP_RABIT · Oryctolagus cuniculus

View on UniProt ↗

Primary accession: P25274
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: MBP
Protein existence: 1: Evidence at protein level
Organism: Oryctolagus cuniculus (Rabbit)
Taxonomy ID: 9986
Alternative names: MBP
EC number: —
Processing: —
Secondary accessions: —

Protein keywords

PTM

AcetylationCitrullinationMethylationPhosphoprotein

Disease

Autoimmune encephalomyelitis

Cellular component

Cell membraneMembrane

Technical term

Direct protein sequencingReference proteome

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Alternative Products

Catalog	Name
367668	MBP (Myelin Basic Protein)
M9758-01Q	Myelin Basic Protein (MBP)
M30349	Myelin Basic Protein MBP
TRV-047074-01	ELISA Kit for Major Basic Protein (MBP)
151940	Major Basic Protein (MBP)
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