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Rat Myelin Basic Protein (MBP) ELISA Kit

CAT: 0013-GTR18853906-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853906-01Size:48 Tests
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Description
Rat Myelin Basic Protein (MBP) ELISA Kit
Product Name Alternative
Myelin_MBP
UniProt
P02688
Reactivity
Rat
Target
MBP
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
0.10 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MBP. You can calculate the concentration of Rat MBP in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10ng/mL

UniProtKB · P02688

Myelin basic protein

MBP_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P02688
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Mbp
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
MBP
EC number
—
Processing
—
Secondary accessions
Q505J1, Q8R4K6, Q9Z1J4, Q9Z1J5, Q9Z1J6
Protein keywords

PTM

AcetylationCitrullinationMethylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Disease

Autoimmune encephalomyelitis

Cellular component

Cell membraneMembrane

Technical term

Direct protein sequencingReference proteome

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