Products for Research Use Only

Mouse Integrin Beta 3 (ITGb3) ELISA Kit

CAT: 0013-GTR18810251-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18810251-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 3 (ITGb3) .
Product Name Alternative
CD61 ELISA Kit, ITG-B3 ELISA Kit, GP3A ELISA Kit, GP3-A ELISA Kit, GpIIIa ELISA Kit, Platelet Membrane Glycoprotein IIIa ELISA Kit
UniProt
O54890
Reactivity
Mouse
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity
12.2 pg/mL
Notes
For research use only.
Applications Notes
Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITGb3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITGb3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITGb3 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
31.25-2000 pg/mL

UniProtKB · O54890

Integrin beta-3

ITB3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
O54890
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Itgb3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q3TZC6
Protein keywords

Technical term

3D-structureReference proteome

Ligand

CalciumMagnesiumMetal-binding

Biological process

Cell adhesion

Cellular component

Cell junctionCell membraneCell projectionMembranePostsynaptic cell membraneSynapse

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Molecular function

IntegrinReceptor