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Human Elastase 3A (ELA3A) ELISA Kit

CAT: 0013-GTR18810098-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18810098-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3A (ELA3A) .
Product Name Alternative
CELA3A ELISA Kit, ELA3 ELISA Kit, Chymotrypsin-Like Elastase Family Member 3A ELISA Kit, Protease E ELISA Kit
UniProt
P09093
Reactivity
Human
Field of Research
Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
13.2 pg/mL
Notes
For research use only.
Applications Notes
Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ELA3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ELA3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ELA3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ELA3A in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
31.25-2000 pg/mL

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