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Human Elastase 3B (ELA3B) ELISA Kit

CAT: 0013-GTR18858281-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18858281-01Size:48 Tests
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Description
Human Elastase 3B (ELA3B) ELISA Kit
Product Name Alternative
CELA3B, CBPP, E1, EL-1, ELA3B, chymotrypsin like elastase family member 3B
UniProt
P08861
Reactivity
Human
Target
ELA3B
Field of Research
Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
0.19 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ELA3B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ELA3B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ELA3B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ELA3B. You can calculate the concentration of Human ELA3B in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20ng/mL

UniProtKB · P08861

Chymotrypsin-like elastase family member 3B

CEL3B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08861
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CELA3B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.21.70
Processing
Precursor
Secondary accessions
B2RE44, P11423, Q5VU28, Q5VU29, Q5VU30
Protein keywords

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Domain

Signal

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