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Human Hemoglobin Beta (HBb) ELISA Kit

CAT: 0013-GTR18810065-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18810065-01Size:48 Tests
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Description
Human Hemoglobin Beta (HBb) ELISA Kit
Product Name Alternative
HB-B ELISA Kit, HBD ELISA Kit, CD113t-C ELISA Kit, Beta Globin ELISA Kit, Spinorphin ELISA Kit
UniProt
P68871
Reactivity
Human
Target
HBb
Field of Research
Cancer Biology
Assay Type
Competitive
Assay Performance Time
2h
Sample Type
Serum, plasma and erythrocyte lysates
Sensitivity
2.82 pg/mL
Notes
For research use only.
Applications Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBb in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Applications
ELISA
Dynamic Range
7.82-500 pg/mL

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