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ELISA Kit for Hemoglobin Beta (HBb)

CAT: 0338-TRV-053430-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0338-TRV-053430-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Product Name Alternative
HB-B; HBD; CD113t-C; Beta Globin; Spinorphin
UniProt
P68871
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Field of Research
Hematology
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemoglobin Beta (HBb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemoglobin Beta (HBb) and unlabeled Hemoglobin Beta (HBb) (Standards or samples) with the pre-coated antibody specific to Hemoglobin Beta (HBb) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemoglobin Beta (HBb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemoglobin Beta (HBb) in the sample.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.
Assay Performance Time
2h
Sample Type
Serum, plasma and erythrocyte lysates
Detection Range
6.17-500ug/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Hemoglobin Beta (HBb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Hemoglobin Beta (HBb) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 2.81ug/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Hemoglobin Beta (HBb) . No significant cross-reactivity or interference between Hemoglobin Beta (HBb) and analogues was observed.
Product Name Synonym
Hemoglobin Beta
Method
Competitive Inhibition
Organism Species
Homo sapiens (Human)

UniProtKB · P68871

Hemoglobin subunit beta

HBB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P68871
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HBB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A4GX73, B2ZUE0, P02023, Q13852, Q14481, Q14510, Q45KT0, Q549N7, Q6FI08, Q6R7N2, Q8IZI1, Q9BX96, Q9UCD6, Q9UCP8, Q9UCP9
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycationGlycoproteinPhosphoproteinS-nitrosylation

Disease

Congenital dyserythropoietic anemiaDisease variantHereditary hemolytic anemia

Ligand

HemeIronMetal-bindingPyruvate

Molecular function

Hypotensive agentVasoactive

Biological process

Oxygen transportTransport

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