ELISA Kit for Hemoglobin Beta (HBb)

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CAS Number

7732-18-5

Product Name Alternative

HB-B; HBD; CD113t-C; Beta Globin; Spinorphin

UniProt

P68871

Applications

Enzyme-linked immunosorbent assay for Antigen Detection.

Field of Research

Hematology

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemoglobin Beta (HBb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemoglobin Beta (HBb) and unlabeled Hemoglobin Beta (HBb) (Standards or samples) with the pre-coated antibody specific to Hemoglobin Beta (HBb) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemoglobin Beta (HBb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemoglobin Beta (HBb) in the sample.

Assay Protocol

1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
And then add 50µ L prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.

Assay Performance Time

Sample Type

Serum, plasma and erythrocyte lysates

Detection Range

6.17-500ug/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Hemoglobin Beta (HBb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Hemoglobin Beta (HBb) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

Sensitivity

The minimum detectable dose of this kit is typically less than 2.81ug/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Format

48T, 96T, 96Tx5, 96Tx10, 96Tx100

Specificity

This assay has high sensitivity and excellent specificity for detection of Hemoglobin Beta (HBb) . No significant cross-reactivity or interference between Hemoglobin Beta (HBb) and analogues was observed.

Product Name Synonym

Hemoglobin Beta

Method

Competitive Inhibition

Organism Species

Homo sapiens (Human)

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number	7732-18-5
PubChem CID	962
IUPAC Name	oxidane
Molecular Formula	H2O
Molecular Weight	18.015
XLogP	-0.5
Topological Polar Surface Area	1
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	0
Heavy Atom Count	1
Formal Charge	0
Complexity	0

SMILES

InChI

InChI=1S/H2O/h1H2

InChIKey

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Chemical Structure

2D Structure

CAS: 7732-18-5

CID: 962

Formula: H2O

MW: 18.015

Interactive 3D Structure

Loading 3D structure...

View on PubChem Raw 3D data

Computed Properties

Property	Value
Molecular Weight	18.015
XLogP3	-0.5
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	0
Exact Mass	18.010564683
Monoisotopic Mass	18.010564683
Topological Polar Surface Area	1 Å²
Heavy Atom Count	1
Formal Charge	0
Complexity	0
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	1
Compound Is Canonicalized	Yes

UniProtKB · P68871

Hemoglobin subunit beta

HBB_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P68871
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: HBB
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: A4GX73, B2ZUE0, P02023, Q13852, Q14481, Q14510, Q45KT0, Q549N7, Q6FI08, Q6R7N2, Q8IZI1, Q9BX96, Q9UCD6, Q9UCP8, Q9UCP9

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycationGlycoproteinPhosphoproteinS-nitrosylation

Disease

Congenital dyserythropoietic anemiaDisease variantHereditary hemolytic anemia

Ligand

HemeIronMetal-bindingPyruvate

Molecular function

Hypotensive agentVasoactive

Biological process

Oxygen transportTransport

Related Products

Catalog	Name
TRV-034805-01	Recombinant Hemoglobin Beta (HBb)
TRV-026221-01	Recombinant Hemoglobin Beta (HBb)
TRV-043091-01	Recombinant Hemoglobin Beta (HBb)
TRV-033086-01	Recombinant Hemoglobin Beta (HBb)
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TRV-035278-01	Recombinant Hemoglobin Beta (HBb)
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TRV-004985-01	Polyclonal Antibody to Hemoglobin Beta (HBb)
TRV-004393-01	Monoclonal Antibody to Hemoglobin Beta (HBb)
TRV-009432-01	Polyclonal Antibody to Hemoglobin Beta (HBb)

Alternative Products

Catalog	Name
140644	Hemoglobin Beta (HBb)
ARP8663-01	Recombinant Hemoglobin Beta (HBb)
318589-01	Hemoglobin subunit beta (HBB)
abx104939-01	Hemoglobin Beta (HBb) Antibody
SHPD494Ov01-01	Native Hemoglobin Beta (HBb)
GTR18224567	HBB/Hemoglobin Beta Antibody
FY-AB50550-01	Anti-Hemoglobin Beta (HBB)