ELISA Kit for Hemoglobin Beta (HBb)

CAT: 0338-TRV-053430-01Size: 24 TestsDry Ice: NoHazardous: No

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CAT#:0338-TRV-053430-01Size:24 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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CAS Number

7732-18-5

Product Name Alternative

HB-B; HBD; CD113t-C; Beta Globin; Spinorphin

UniProt

P68871

Applications

Enzyme-linked immunosorbent assay for Antigen Detection.

Field of Research

Hematology

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemoglobin Beta (HBb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemoglobin Beta (HBb) and unlabeled Hemoglobin Beta (HBb) (Standards or samples) with the pre-coated antibody specific to Hemoglobin Beta (HBb) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemoglobin Beta (HBb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemoglobin Beta (HBb) in the sample.

Assay Protocol

1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
And then add 50µ L prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.

Assay Performance Time

Sample Type

Serum, plasma and erythrocyte lysates

Detection Range

6.17-500ug/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Hemoglobin Beta (HBb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Hemoglobin Beta (HBb) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

Sensitivity

The minimum detectable dose of this kit is typically less than 2.81ug/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Format

48T, 96T, 96Tx5, 96Tx10, 96Tx100

Specificity

This assay has high sensitivity and excellent specificity for detection of Hemoglobin Beta (HBb) . No significant cross-reactivity or interference between Hemoglobin Beta (HBb) and analogues was observed.

Product Name Synonym

Hemoglobin Beta

Method

Competitive Inhibition

Organism Species

Homo sapiens (Human)

UniProtKB · P68871

Hemoglobin subunit beta

HBB_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P68871
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: HBB
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: A4GX73, B2ZUE0, P02023, Q13852, Q14481, Q14510, Q45KT0, Q549N7, Q6FI08, Q6R7N2, Q8IZI1, Q9BX96, Q9UCD6, Q9UCP8, Q9UCP9

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycationGlycoproteinPhosphoproteinS-nitrosylation

Disease

Congenital dyserythropoietic anemiaDisease variantHereditary hemolytic anemia

Ligand

HemeIronMetal-bindingPyruvate

Molecular function

Hypotensive agentVasoactive

Biological process

Oxygen transportTransport

Related Products

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TRV-034805-01	Recombinant Hemoglobin Beta (HBb)
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Alternative Products

Catalog	Name
140644	Hemoglobin Beta (HBb)
ARP8663-01	Recombinant Hemoglobin Beta (HBb)
318589-01	Hemoglobin subunit beta (HBB)
SHPD494Ov01-01	Native Hemoglobin Beta (HBb)
abx104939-01	Hemoglobin Beta (HBb) Antibody
GTR18224567	HBB/Hemoglobin Beta Antibody
FY-AB50550-01	Anti-Hemoglobin Beta (HBB)