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Human Complement Factor I (CFI) ELISA Kit

CAT: 0013-GTR18808894-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18808894-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor I (CFI) .
Product Name Alternative
CF-I ELISA Kit, C3b-INA ELISA Kit, FI ELISA Kit, IF ELISA Kit, KAF ELISA Kit, Factor I ELISA Kit, Konglutinogen-Activating Factor ELISA Kit, C3b-Inactivator ELISA Kit, C3B/C4B inactivator ELISA Kit
UniProt
P05156
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
0.072 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFI in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL

UniProtKB · P05156

Complement factor I

CFAI_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P05156
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CFI
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.21.45
Processing
Precursor
Secondary accessions
O60442
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Age-related macular degenerationDisease variantHemolytic uremic syndrome

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Biological process

Complement pathwayHost-virus interactionImmunityInnate immunity

Molecular function

HydrolaseProteaseSerine protease

Domain

RepeatSignal

Cellular component

Secreted

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