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Rat Interferon Beta (IFNb) ELISA Kit

CAT: 0013-GTR18808968-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18808968-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Beta (IFNb) .
Product Name Alternative
IFNB1 ELISA Kit, IFN-B ELISA Kit, IFB ELISA Kit, IFF ELISA Kit, IFNB ELISA Kit, Interferon Beta 1 Fibroblast, IFN Beta ELISA Kit
UniProt
P70499
Reactivity
Rat
Field of Research
Infectious Disease & Virology; Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
2.9 pg/mL
Notes
For research use only.
Applications Notes
Standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IFNb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IFNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IFNb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IFNb in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
7.82-500 pg/mL

UniProtKB · P70499

Interferon beta

IFNB_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P70499
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ifnb1
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
IFN-beta
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Antiviral defense

Molecular function

Cytokine

PTM

Disulfide bondGlycoproteinPhosphoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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