Human Interleukin 2 (IL2) ELISA Kit

CAT: 0013-GTR18807557-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0013-GTR18807557-01Size:48 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 (IL2) .

Product Name Alternative

TCGF ELISA Kit, Lymphokine ELISA Kit, T-Cell Growth Factor ELISA Kit, Aldesleukin ELISA Kit

UniProt

P60568

Reactivity

Human

Field of Research

Immunology & Inflammation

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

5.9 pg/mL

References & Citations

D Siedlecka, L Bielawska, A Ludziejewska, A Baszczuk, E Wysocka IL-2 and IL-7 Contribution to Immune Response: Effects of Vaccination Against COVID-19 in Adults Viruses, (2025)

Notes

For research use only.

Applications Notes

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL2 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

15.63-1000 pg/mL

UniProtKB · P60568

Interleukin-2

IL2_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P60568
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: IL2
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: IL-2
EC number: —
Processing: Precursor
Secondary accessions: P01585

Protein keywords

Technical term

3D-structureDirect protein sequencingPharmaceuticalProteomics identificationReference proteome

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Chromosomal rearrangement

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoprotein

Disease

Proto-oncogene

Cellular component

Secreted

Domain

Signal

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