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Human Apolipoprotein E (APOE) ELISA Kit

CAT: 0013-GTR18808268-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18808268-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein E (APOE) .
Product Name Alternative
Apo-E ELISA Kit, AD2 ELISA Kit, Apoprotein ELISA Kit, Alzheimer Disease 2 (E4-Associated, Late Onset ELISA Kit
UniProt
P02649
Reactivity
Human
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
1.8 ng/mL
Notes
For research use only.
Applications Notes
Standard: 300 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOE in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
4.69-300 ng/mL

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