Suc-AAPF-pNA

• UNSPSC Description:

  Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a chromogenic p-nitroanilide (pNA) substrate with the Km of 1.7 mM. Cleavage of Suc-AAPF-pNA releases 4-nitroaniline, which is yellow in colour and can be measured spectrophotometrically. Suc-AAPF-pNA can be used for the measurement of free and membrane-bound cathepsin G in human neutrophils[1][2][3].

• Target Antigen:

  Others

• Type:

  Peptides

• Related Pathways:

  Others

• Applications:

  COVID-19-immunoregulation

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/suc-aapf-pna.html

• Purity:

  99.87

• Solubility:

  DMSO : 100 mg/mL (ultrasonic)

• Smiles:

  OC(CCC(N[C@@H](C)C(N[C@@H](C)C(N1[C@@H](CCC1)C(N[C@H](C(NC2=CC=C(C=C2)[N+]([O-])=O)=O)CC3=CC=CC=C3)=O)=O)=O)=O)=O

• Molecular Weight:

  624.64

• References & Citations:

  [1]Sylvie Attucci, et al. Measurement of free and membrane-bound cathepsin G in human neutrophils using new sensitive fluorogenic substrates. Biochem J. 2002 Sep 15;366(Pt 3):965-70.|[2]A J Webb, et al. A protease-based biosensor for the detection of schistosome cercariae. Sci Rep. 2016 Apr 19:6:24725. |[3]Jeanette M Mucha, et al. Enhanced immunogenicity of a functional enzyme by T cell epitope modification. BMC Immunol. 2002:3:2.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  -80°C, 2 years; -20°C, 1 year (Powder, sealed storage, away from moisture and light, under nitrogen)

• Clinical Information:

  No Development Reported

• CAS Number:

  70967-97-4